Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells
Identifieur interne : 000090 ( Main/Exploration ); précédent : 000089; suivant : 000091Evaluation of A(H1N1)pdm09 LAIV vaccine candidates stability and replication efficiency in primary human nasal epithelial cells
Auteurs : Svetlana Shcherbik [États-Unis] ; Nicholas Pearce [États-Unis] ; Paul Carney [États-Unis] ; Ekaterina Bazhenova [Russie] ; Natalie Larionova [Russie] ; Irina Kiseleva [Russie] ; Larisa Rudenko [Russie] ; Amrita Kumar [États-Unis] ; Cynthia S. Goldsmith [États-Unis] ; Vivien Dugan [États-Unis] ; James Stevens [États-Unis] ; David E. Wentworth [États-Unis] ; Tatiana Bousse [États-Unis]Source :
- Vaccine: X [ 2590-1362 ] ; 2019.
Abstract
The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component. In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus. We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN). Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13). Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference. Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.
Url:
DOI: 10.1016/j.jvacx.2019.100031
PubMed: 31384746
PubMed Central: 6668239
Affiliations:
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Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="af010">Battelle, Atlanta, GA 30329, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Battelle, Atlanta, GA 30329</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. Goldsmith</name><affiliation wicri:level="2"><nlm:aff id="af020">Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Dugan, Vivien" sort="Dugan, Vivien" uniqKey="Dugan V" first="Vivien" last="Dugan">Vivien Dugan</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. 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States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="af010">Battelle, Atlanta, GA 30329, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Battelle, Atlanta, GA 30329</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Carney, Paul" sort="Carney, Paul" uniqKey="Carney P" first="Paul" last="Carney">Paul Carney</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bazhenova, Ekaterina" sort="Bazhenova, Ekaterina" uniqKey="Bazhenova E" first="Ekaterina" last="Bazhenova">Ekaterina Bazhenova</name><affiliation wicri:level="1"><nlm:aff id="af015">Institute of Experimental Medicine, Department of Virology, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Department of Virology, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Larionova, Natalie" sort="Larionova, Natalie" uniqKey="Larionova N" first="Natalie" last="Larionova">Natalie Larionova</name><affiliation wicri:level="1"><nlm:aff id="af015">Institute of Experimental Medicine, Department of Virology, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Department of Virology, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Kiseleva, Irina" sort="Kiseleva, Irina" uniqKey="Kiseleva I" first="Irina" last="Kiseleva">Irina Kiseleva</name><affiliation wicri:level="1"><nlm:aff id="af015">Institute of Experimental Medicine, Department of Virology, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Department of Virology, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name><affiliation wicri:level="1"><nlm:aff id="af015">Institute of Experimental Medicine, Department of Virology, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Experimental Medicine, Department of Virology, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Amrita" sort="Kumar, Amrita" uniqKey="Kumar A" first="Amrita" last="Kumar">Amrita Kumar</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="af010">Battelle, Atlanta, GA 30329, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Battelle, Atlanta, GA 30329</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. Goldsmith</name><affiliation wicri:level="2"><nlm:aff id="af020">Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Dugan, Vivien" sort="Dugan, Vivien" uniqKey="Dugan V" first="Vivien" last="Dugan">Vivien Dugan</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name><affiliation wicri:level="2"><nlm:aff id="af005">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine: X</title><idno type="eISSN">2590-1362</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The recent reduction of live attenuated influenza vaccine (LAIV) effectiveness in multivalent formulations was particularly associated with the A(H1N1)pdm09 component. In the 2017 the WHO vaccine composition committee changed its recommendations for the A(H1N1)pdm09 component to include an A/Michigan/45/2015-like virus. We evaluated effectiveness and quality of newly developed and previous A(H1N1)pdm09 LAIV reassortants through assessment of their thermal and pH stability, receptor binding specificity and replication fitness in primary human airway epithelial cells of nasal origin (hAECN). Our analysis showed that LAIV expressed hemagglutinin (HA) and neuraminidase (NA) from an A/Michigan/45/2015-like strain A/New York/61/2015 (A/New York/61/2015-CDC-LV16A, NY-LV16A), exhibit higher thermal and pH stability compared to the previous vaccine candidates expressing HA and NA from A/California/07/2009 and A/Bolivia/559/2013 (A17/Cal09 and A17/Bol13). Reassortants A/South Africa/3626/2013-CDC-LV14A (SA-LV14A) and NY-LV16A showed preferential binding to α2,6 sialic acid (SA) receptors and replicated at higher titers and more extensively in hAECN compared to A17/Cal09 and A17/Bol13, which had an α2,3 SA receptor binding preference. Our data analysis supports selection of A/New York/61/2015-CDC-LV16A for LAIV formulation and the introduction of new assays for LAIV characterization.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J. Liu</name></author><author><name sortKey="Kemble, G" uniqKey="Kemble G">G. Kemble</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. 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Jarvis</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Russie</li><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name></region><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name><name sortKey="Carney, Paul" sort="Carney, Paul" uniqKey="Carney P" first="Paul" last="Carney">Paul Carney</name><name sortKey="Dugan, Vivien" sort="Dugan, Vivien" uniqKey="Dugan V" first="Vivien" last="Dugan">Vivien Dugan</name><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. Goldsmith</name><name sortKey="Kumar, Amrita" sort="Kumar, Amrita" uniqKey="Kumar A" first="Amrita" last="Kumar">Amrita Kumar</name><name sortKey="Kumar, Amrita" sort="Kumar, Amrita" uniqKey="Kumar A" first="Amrita" last="Kumar">Amrita Kumar</name><name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name><name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name></country><country name="Russie"><noRegion><name sortKey="Bazhenova, Ekaterina" sort="Bazhenova, Ekaterina" uniqKey="Bazhenova E" first="Ekaterina" last="Bazhenova">Ekaterina Bazhenova</name></noRegion><name sortKey="Kiseleva, Irina" sort="Kiseleva, Irina" uniqKey="Kiseleva I" first="Irina" last="Kiseleva">Irina Kiseleva</name><name sortKey="Larionova, Natalie" sort="Larionova, Natalie" uniqKey="Larionova N" first="Natalie" last="Larionova">Natalie Larionova</name><name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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